中德临床肿瘤学杂志（英文版）2009，Vol.8Issue(2)：100-103,4.DOI:10.1007/s10330-008-0097-9
FLT3/ITD基因突变在不同血液肿瘤患者的表达及其临床意义
Detection of FLT3/ITD gene mutations in patients with hematologic malignancy and their clinical Significance
摘要
Abstract
Objective:To analyze Fms-like tyrosine kinase 3(FLT3)/intemal-tandem duplications(ITD)murations in various kinds of hematologic malignancy patients.Methods:FLT3/ITD gene mutations were detected by polymerase chain reaction (PCR)in 103 acute myeloid leukemia(AML)cases,63 acute lymphocytic leukemia(ALL)cases,53 chronic myelogenous leukemia(CML)cases in chronic phase(CML-CP),34 CML cases in biast crisis(CML-BC),11 chronic lymphatic leukemia(CLL)cases,36 myelodysplastic syndrome (MDS)cases,9 multiple myeloma(MM)cases and 13 non-hodgkin's lymphoma (NHL)cases with marrow infiltration.Results:The expressions of FLT3/ITD gene mutations were detected in 22.3% AML cases.in 6.5%CML-BC cases.in 5.6%MDS cases and in 2.6%ALL cases.The two ALL cases with FLT3/ITD mutation were diagnosed as ALL-L2 with morphology and both with myeloid antigen expression,but finally were diagnosed as acute mixed-lineage leukemia after immunology examination.FLT3/ITD gene muIations were not detected in CML-CP,MM.NHL and CLL cases.In the 23 AML patients with FLT3/ITD gene mutation,including 2 of 8 M1(2.5%),8 of 33 M2(24-2%),7 of 24 M3(29.3%),2 of 11 M4(18.2%).3 of 21 M5(14.3%),1 of 5 M6(20%),and 0 of 1 M7 cases,and there were no significant differences in the positive rates of FLT3/ITD mutations between the FAB subtypes(P＞0.05).Statistical analyses showed that in AML patients,FLT3/ITD was associated with a higher pefipheral blood white cell(WBC)counts[(41.23±32.56)x 100/L vs (11.36±9.89)x109/L(P＜0.01)],higher percentage of bone marrow blast cells[(72.78±21.79)%vs(51.26±20.78)%(P＜0.05)],and higher cumulative relapse rates(63.6%vs 27.7%,P＜0.025)than those negative.Conclusion:FLT3/ITD gene mutation mainly pccurred in AML patients.and might be a strong prognostic factor which was associated with high peripheral WBC counts.bone marrow blast cell proportion and a increased relapse risk in AML.Detection of FLT3/ITD gene mutation might provide insights to explore a more accurate genotyping of leukemia,differential diagnosis between AML and ALL.subdivide risk level in AML and estimate prognosis of leukemia.关键词
FLT3/ITD mutation/hematologic malignancy/polymerase chain reaction (PCR)/acute myeloid leukemia(AML)Key words
FLT3/ITD mutation/hematologic malignancy/polymerase chain reaction (PCR)/acute myeloid leukemia(AML)分类
临床医学引用本文复制引用
Bing Xu,Guoshu Chen,Xiaodan Luo,Jiahong Tang..FLT3/ITD基因突变在不同血液肿瘤患者的表达及其临床意义[J].中德临床肿瘤学杂志（英文版）,2009,8(2):100-103,4.基金项目
Supported by a grant from the Technology Program of Guangdong Province(No.2002C30304). （No.2002C30304）
