中国临床药理学与治疗学2004，Vol.9Issue(9)：1002-1006,5.
高效液相色谱-质谱联用测定人体尼索地平血浆浓度及其药代动力学研究
Determination of concentration of nisoldipine in human plasma by HPLC-MS method and investigation of its pharmacokinetics
郭瑞臣 1魏春敏 2王本杰1
作者信息
- 1. 山东大学齐鲁医院临床药理研究所,济南,250012,山东
- 2. 山东大学齐鲁医院临床药理研究所,药学系,济南,250012,山东
- 折叠
摘要
Abstract
AIM: To determine the concentration of nisoldipine in human plasma by HPLC-MS method and investigate the pharmacokinetics of sustained and immediate-release preparations. METHODS: A C18 column was used to separate nisoldipine from plasma with the mobile phase of a mixture of methanol-water-acetic acid (75: 25ic pressure electronic spray ionization (AP-ESI) and ion mass spectral (m/z) of 411 were selected to quantify nisoldipine. Internal standard (IS): atmospheric pressure electronic spray ionization and m/z of 441 for nimodipine.RESULTS: The linear range of the standard curve of ni70%, and intra-day relative standard deviation (RSD)and inter-day RSD were less than 10%. After being given a single dose of 10 mg nisoldipine sustained release tablet, sustained release capsule and normal tablet, the half life(t1/2/h) were 6.08 ± 1.48, 7.06 ± 1.80 and 3.70± 0.25, the time to peak concentration (Tpeak/h)were 5.4 ± 0.7, 5.8 ± 0.4 and 2.0 ± 0.2, the peak 0.24 and 9.18 ± 3.78, the area under time- concentra33.63 ± 7.16 and 32.72 ± 5.09. But after being given 0.72 ± 0.10, 0. 77 ± 0.07 and 0.53 ± 0.07, DF were 175.00% ± 16.34%, 177.10% ± 18.43% and 247.92% ± 57.71% respectively. The bioavailability of sustained- release tablet and capsule were 96% ± 12%and 102% ± 9% respectively. CONCLUSION: The determination of concentration of nisoldipine in human plasma by HPLC-MS method is sensitive and accurate. It can be used for the investigation of the bioavailability and pharmacokinetic of nisoldipine.关键词
尼索地平/尼莫地平/高效液相色谱-质谱法:生物利用度/药动学Key words
nisoldipine/nimodipine/HPLC-MS/bioavailability/pharmacokinetics分类
医药卫生引用本文复制引用
郭瑞臣,魏春敏,王本杰..高效液相色谱-质谱联用测定人体尼索地平血浆浓度及其药代动力学研究[J].中国临床药理学与治疗学,2004,9(9):1002-1006,5.
