广东医学2001，Vol.22Issue(2)：101-103,3.
人气道上皮细胞内皮素转换酶-1反义RNA构建
Construction of endothelin converting enzyme-1 antisense RNA expression vector from human bronchial epithelial cells
摘要
Abstract
Objective To explore the effects of endothelin converting enzyme-1 (ECE-1) antisense RNA on ECE-1 expression in human bronchial epithelial cell line(16HBE). Methods ECE-1 antisense RNA expression vector with green fluorescent protein as a reporter was constructed and transfected into 16HBE with Fugene 6TM.The ECE-1 expression was measured by RT-PCT and Western bloting. Results Agrose gel electrophoresie showed that 0.717kp ECE DNA fragment was inserted into pEGFPC1 plasmid in reverse orientation. After G418 selection, anti-16HBE cell line stably expressing ECE-1 antisense RNA was developed. Both ECE-1 mRNA(ECE/actin β 0.012 5±0.058) and protein expression in anti-16HBE cells were significantly lower than those in normal cells(ECE/actin β 0.134 7±0.067) detected by RT-PCR and Western bloting respectively(P＜0.05). Conclusion ECE-1 expression in 16HBE cells can be inhibited by ECE-1 antisense RNA in vitro.关键词
内皮素转换酶核糖核酸反义载体构建分类
医药卫生引用本文复制引用
文思成,徐军,任筱兰,钟南山..人气道上皮细胞内皮素转换酶-1反义RNA构建[J].广东医学,2001,22(2):101-103,3.基金项目
广东省自然科学基金资助项目(编号：980456) （编号：980456）
