中国癌症防治杂志2009，Vol.1Issue(2)：101-104,4.
抑制CDC25A表达的siRNA真核表达载体的构建
Construction of eukaryotic expression vector of siRNA-inhibited CDC25A expression
摘要
Abstract
Objective To construct and identify an eukaryotic expression vector of siRNA-inhibited CDC25A expression in mammalian cells.Methods CDC25A-specific siRNA was designed and synthesized according to the sequence of CDC25A.After annealing the nucleic acid fragment of the siRNA to form the double-stranded, they were connected with the eukaryotic expression vector psilencer4.1 which was pre-digested with Bam HI and Hind III.Then the constructed vectors, named psilencer4.1-CDC25A and psilencer4.1-Control, were digested and sequenced.Results The vector of siRNA-inhibited CDC25A expression and the negative control vector were constructed, sequenced and identified.Conclusion The successful construction and validation of the siRNA-inhibited CDC25A expression vector lays a good foundation for the next steps in CDC25A-related study.关键词
CDC25A/siRNA/载体Key words
CDC25A/siRNA/Vector分类
医药卫生引用本文复制引用
朱小东,李相德,曲颂,李龄,黎丹戎,张玮,李力,唐步坚..抑制CDC25A表达的siRNA真核表达载体的构建[J].中国癌症防治杂志,2009,1(2):101-104,4.基金项目
广西医疗卫生重点科研课题(重200866) （重200866）
广西科学研究与技术开发计划项目(桂科攻0592007-1B) （桂科攻0592007-1B）
广西研究生教育创新计划项目(2007105981002M 19) （2007105981002M 19）
