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Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

WANG Wei-juan CHEN Yu-zhen LIU Mei-qin LU Cun-fu

中国林学(英文版)2008,Vol.10Issue(2):101-106,6.
中国林学(英文版)2008,Vol.10Issue(2):101-106,6.

Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings

WANG Wei-juan 1CHEN Yu-zhen 1LIU Mei-qin 1LU Cun-fu1

作者信息

  • 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China
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摘要

Abstract

Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance. In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus, seedlings were acclimated at 2℃ under 16-h photoperiod (150 μmol·m-2·s-1 photosynthetically active radiation) for 14 d. Freezing tolerance in seedlings increased after 14 d of cold-hardening. Contents of protein, proline and solute carbohydrate in cotyledon increased after cold acclimation. Patterns of isozymes of superoxide dismutase (SOD), peroxidase, catalase and polyphenol oxidase (PPO) were investigated. The activities of SOD, peroxidase and PPO in cold acclimated plants were increased during cold-hardening. We deduced that compatible solutes and antioxidant enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant.

关键词

Ammopiptanthus mongolicus, cold acclimation, freezing tolerance, compatible solute, antioxidant enzyme

Key words

Ammopiptanthus mongolicus, cold acclimation, freezing tolerance, compatible solute, antioxidant enzyme

分类

农业科技

引用本文复制引用

WANG Wei-juan,CHEN Yu-zhen,LIU Mei-qin,LU Cun-fu..Effects of cold-hardening on compatible solutes and antioxidant enzyme activities related to freezing tolerance in Ammopiptanthus mongolicus seedlings[J].中国林学(英文版),2008,10(2):101-106,6.

基金项目

This work was financially supported by the National Natural Science Foundation of China (Grant Nos.30671476 and 30271067). (Grant Nos.30671476 and 30271067)

中国林学(英文版)

2095-6355

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