中国动脉硬化杂志2001，Vol.9Issue(1)：10-13,4.
氧化型低密度脂蛋白对大鼠血管平滑肌细胞基质金属蛋白酶-2和9表达的影响
Effects of Oxidized Low Density Lipoprotein on Expression of Matrix Metalloproteinase-2 and 9 in Cultured Rat Vascular Smooth Muscle Cells
摘要
Abstract
Aim To examine the effects of oxidized low density lipoprotein(ox-LDL) on expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 in cultured rat vascular smooth muscle cells (VSMCs), and explore the relation between the migration and proliferation of VSMCs and the expression activity of these two genes. Methods Northern blot, Dot blot, Western blot and SDS-PAGE containing gelatin were used to detect MMP-2 and MMP-9 mRNA expression, protein synthesis and enzyme activities. Results The mRNA expressions, protein levels and enzyme activities of MMP-2 and MMP-9 significantly increased after VSMCs were stimulated by 10 mg/L ox-LDL for 24 h. When the concentration of ox-LDL were enhanced to 50 mg/L, different effects were exerted on MMP-2 and MMP-9. The levels of MMP-9 were still higher than control group and as the same as the group of 10 mg/L ox-LDL. However, the expression of MMP-2 decreased markedly at the same condition. Conclusion Ox-LDL could increase the expression activities of MMP-2 and MMP-9 and the degradation of ECM.关键词
肌,平滑,血管/脂蛋白,低密度/金属蛋白酶/基因表达/大鼠分类
生物科学引用本文复制引用
刘虹彬,温进坤,韩梅..氧化型低密度脂蛋白对大鼠血管平滑肌细胞基质金属蛋白酶-2和9表达的影响[J].中国动脉硬化杂志,2001,9(1):10-13,4.基金项目
国家自然科学基金（39770312）及河北省自然科学基金 （）
