中国林学（英文版）2006，Vol.8Issue(4)：10-14,5.
Establishment of in vitro culture of Populus euphratica Olivier
Establishment of in vitro culture of Populus euphratica Olivier
摘要
Abstract
The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP2. In optimizing media for in vitro plant cultures including MS, B5 and MP2 media we employed hormones, auxin IAA, cytokine benzyladenine (BAP) and gibberellic acid (GA) in our factorial experiments on media. Adventitious shoots were derived from cuttings of adult plants taken from Xingjiang, west China, on selected media with MP2 + 0.5 mg.L-1 BA + 0.1 mg·L-1 NAA. The shoots were elongated on a medium with 0.25mg.L-1 BAP, 0.1 mg.L-1NAA and 2 mg·L-1 GA and were then rooted on a medium with 0.2-0.5 mg·L-1 IBA. All the media were incorporated with 30 g·L-1 sucrose and an adjusted pH at 6.3.关键词
Populus euphratica Olivier/plant hormones/in vitro culture/micropropagationKey words
Populus euphratica Olivier/plant hormones/in vitro culture/micropropagation分类
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Zhao Peng,Dong Zhan-yuan,Sun Hong-bin,Zhao Ju-ying,Wang Hua-fang..Establishment of in vitro culture of Populus euphratica Olivier[J].中国林学（英文版）,2006,8(4):10-14,5.基金项目
This study was financially supported by the National Natural Science Foundation of China (Grant No.30371148). （Grant No.30371148）
