首页|期刊导航|中国农业科学（英文版）|Study on Red Coat Color Gene and Prediction of the Secondary Structure in Chinese Holstein
中国农业科学（英文版）2008，Vol.7Issue(8)：1016-1021,6.
Study on Red Coat Color Gene and Prediction of the Secondary Structure in Chinese Holstein
Study on Red Coat Color Gene and Prediction of the Secondary Structure in Chinese Holstein
摘要
Abstract
The nucleotide sequence of the melanocortin-1-receptor (MC1R) gone was studied with the help of the polymerase chain reaction (PCR), in which the protein structure in Chinese Holstein was predicted, and the molecular mechanism of the red coat color was investigated. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to genotype the individuals. The bioinformatics and biotechnology softwares were used to predict the secondary structure of MC1R. The results showed that the EE genotype was the dominant genotype in Chinese Holstein Black and White herd, whereas, it was ee in Chinese Holstein Red and White herd. The secondary structure of the mutational MCIR protein was changed and the deletion mutation caused an earlier termination in translation, which led to the formation of the red coat color. The allele E was mainly associated with the black coat color, whereas, e was associated with red.关键词
bovine/red coat color/MCIR/protein structureKey words
bovine/red coat color/MCIR/protein structure分类
农业科学引用本文复制引用
..Study on Red Coat Color Gene and Prediction of the Secondary Structure in Chinese Holstein[J].中国农业科学（英文版）,2008,7(8):1016-1021,6.基金项目
This study was supposed by National 863 Program of China(2006AAl02lD9,2007AAl02169),and High Technology Independent Innovation Project from Shandong ACademy of Agricultural Sciences (2006YCX028),and Youth Science Foundation from Shandong Academy of Agricultural Sciences of China(2006YON038). （2006AAl02lD9,2007AAl02169）
