河南农业科学Issue(7)：102-106,5.
树型金银花染色体制片优化及核型分析
Optimization of Chromosome Mounting Technique and Karyotype Analysis of Tree Lonicera japonica
摘要
Abstract
It was compared that the effects of different sampling time,the methods of pretreatments,dissociations time and dyeing time on chromosome preparation of tree Lonicera japonica shoot tips.The results showed that the best experimental protocol for karyotype analysis of tree Lonicera japonica.is better when sampling time is between 10:00AM and 11:00AM,the pretreatment time with saturated p-dichlorobenzene lasts 3-4h,the dissociation with 1mol/L HCl is at 60℃ for 13min,and the dyeing time lasts 10min with modified carbolic acid-magenta.Simultaneously, the karyotype analysis also showed that the karyotype formulae of tree Lonicera japonica is 2n=18= 8m +8sm+2st,where one satellite was found in the ninth chromosomes, the relative length of the chromosome composition was 2n=18=4L+8M2+4M1+2S, belonging to "2B" type.Its index of Asymmetric was 64.47%, and the karyotype asymmetry belongs to transition type from symmetry type to asymmetry one, suggesting that tree Lonicera japonica is relatively evolutionary form compared to wild type Lonicera japonica.关键词
树型金银花/染色体制片/核型分析Key words
Tree Lonicera japonica/Chromosome preparation/Karyotype analysis分类
农业科技引用本文复制引用
李晓玲,杨进,王洪涛,李宁,汪祥..树型金银花染色体制片优化及核型分析[J].河南农业科学,2009,(7):102-106,5.基金项目
湖北省科技攻关项目(2005AA201C53) （2005AA201C53）
宜昌市科技攻关项目(A2007103-05) （A2007103-05）
