生命科学研究2001，Vol.5Issue(2)：102-108,7.
大规模动物细胞培养技术研究进展
Progress in Technology for Scale-up Culture of Animal Cells
邹寿长 1李干祥 2杨葆生 2徐秀英3
作者信息
- 1. 中信股份有限公司,
- 2. 深圳市斯贝克生物药业有限公司,
- 3. 军事医学科学院生物工程研究所,
- 折叠
摘要
Abstract
Many biological products were manufactur ed by means of large-up culture of animal cells. To increase the cell-specific productivity and to facilitate purification, serum-free media supplemented wit h several nutriments were used for cell culture, and microcarriers were chosen in favor of cell growth and high cell density. Bioreactors with simple manipula tion, good qualification and aeration were adopted. More suitable conditions fo r cell growth could be given, and higher expression level could be gained thro ugh on-line supervising the environment for cell growth and decreasing restrain t factors in cell culturing. The expression of anti-apoptosis genes using recom binant DNA technology could increase cell viability and productivity. The beads -to-beads transfer method of porous microcarriers was emphasized in large-up culture of animal cells.关键词
细胞培养/生物反应器/动物细胞/微载体分类
生物科学引用本文复制引用
邹寿长,李干祥,杨葆生,徐秀英..大规模动物细胞培养技术研究进展[J].生命科学研究,2001,5(2):102-108,7.
