分析化学2009，Vol.37Issue(7)：1025-1028,4.
超高效纳升液相色谱-电喷雾串联质谱鉴定重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白
Characterization of Recombinant Human Necrosis Factor-α ReceptorⅡ:IgG Fc Fusion Protein by Nano Ultra-high Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry Tandem Mass Spectrometry
摘要
Abstract
The Nano ultra-high performance liquid chromatography-electrospray ionization mass spectrometry tandem mass spectrometry(UPLC-ESI-MS/MS) was used to characterize the primary protein. Samples were digested by trypsin, followed by liquid chromatography-tandem mass spectrometry analysis and database search. Five peptides matched with tumor necrosis factor receptor and seven peptides matched with human IgG1 fragment of the Fc. Further analysis demonstrated that seven peptides all matched with the IgG1 Fc but only partly peptides matched with the other subtypes. RhTNFR:Fc is fused by IgG1 Fc but not other subtypes.关键词
基因重组受体-抗体融合蛋白/纳升液相色谱/电喷雾串联质谱/蛋白鉴定Key words
Recombinant human necrosis factor-α receptorⅡ∶ IgG Fc fusion protein, nano ultra-high perfor-mance liquid chromatography-electrospray ionization mass spectrometry tandem mass spectrometry, protein identification分类
化学化工引用本文复制引用
柏兆方,林碧蓉,李萍,李卫华,刘炳玉,王红霞..超高效纳升液相色谱-电喷雾串联质谱鉴定重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白[J].分析化学,2009,37(7):1025-1028,4.基金项目
本文系"十一五"国家科技支撑计划(No.2006BAF07B01)资助 （No.2006BAF07B01）
