首页|期刊导航|中华医学杂志（英文版）|Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer
中华医学杂志（英文版）2003，Vol.116Issue(7)：1029-1033,5.
Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer
Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer
摘要
Abstract
Objective To evaluate the effects of hepatocyte growth factor (HGF) on the prevention of scar formation and the promotion of wound healing by gene transfer. Methods A total of 12 female New Zealand rabbits were used in this study. Rabbits were anesthetized with an intravenous injection of sodium pentobarbital, and identical wounds were made over the ventral surface of each ear. Five circular wounds, 7 mm in diameter, were created in each ear by excision through the skin to the underlying cartilage using sterile technique. After the surgical procedures, 10 of the rabbits were randomly allocated to five groups, with 2 rabbits in each group: Ad-HGF group 1, Ad-HGF group 2, Ad-HGF group 3, Ad-GFP (a reporter gene) group and the solvent group. Immediately after surgery, 6×107 pfu Ad-HGF, 6×108 pfu Ad-HGF, 6×109 pfu of Ad-HGF, 6×109 pfu of Ad-GFP, or same volume of solvent (PBS, pH 7.2) was applied once to each wound in groups 1 to 5, respectively. One additional rabbit was used to evaluate the transfer efficiency of the adenovirus vector by transferring Ad-GFP (6×109 pfu) into its wounds. Ice slides of wounds from this animal were observed under fluorescence microscopy. Another additional rabbit was used to evaluate the expression of HGF and TGFβ1 after transferring Ad-HGF (6×109 pfu) into each of its wound. Immunohistochemistry was used for detection. Results The effect of HGF on reducing excessive dermal scarring was observed by adenovirus-mediated gene transfer. Transfection of the human HGF cDNA into skin wounds through an adenoviral vector suppressed the over-expression of TGFβ1, which plays an essential role in the progression of dermal fibrogenesis. Application of HGF to the wounds significantly enhanced wound healing and inhibited over scarring.Conclusion HGF gene therapy could be a new approach for preventing excessive dermal scarring in wound healing.关键词
scar/wound healing/hepatocyte growth factor/gene transfer/adenoviral vectorKey words
scar/wound healing/hepatocyte growth factor/gene transfer/adenoviral vector分类
医药卫生引用本文复制引用
哈小琴,李元敏,劳妙芬,苑宾,吴祖泽..Effect of human hepatocyte growth factor on promoting wound healing and preventing scar formation by adenovirus-mediated gene transfer[J].中华医学杂志（英文版）,2003,116(7):1029-1033,5.基金项目
This work was supported by the State High-Technology Department Program (No. 2001AA217061) and partly by a grant from the Biological Engineering Department, Far East Area of American Baxter Healthcare Products Co. Ltd., USA. （No. 2001AA217061）
