华东理工大学学报（自然科学版）2001，Vol.27Issue(1)：103-105,3.
珍珠角壳蛋白中氨基酸的高效液相色谱分析
Determination of Amino Acids in Protein of Pearls by Reversed-phase High Performance Liquid Chromatography
摘要
Abstract
By use of octadecyl (C18) column made in China, a RP-HPLC method was studied using phenylisothiocyanate (PITC) as the pre-column derivatizing reagent for the determination of amino acids. Under room temperature, pH6.3 HAc-NaAc buffers, acetonitrile-water solutions as the mobile phase and the use of the gradient elution mode, fine resolution of phenylthiocarbamyl derivatives of 18 kinds of common amino acids was achieved after about 30 minutes and the content of amino acids (except Trp) in the hydrolysate of protein of pearl was determined. The results showed that the proteins in pearls mainly consist of 10 kinds of amino acids such as Asp, Glu, Ser, Gly, Ala, Leu and so on which account for about 85 percent of total amount of protein.关键词
珍珠/蛋白质/氨基酸/异硫氰酸苯酯/反相高效液相色谱分类
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金凤明,冯学伟,李桂贞..珍珠角壳蛋白中氨基酸的高效液相色谱分析[J].华东理工大学学报（自然科学版）,2001,27(1):103-105,3.
