首页|期刊导航|中华医学杂志（英文版）|Effect of trans-acting factor on rat glutathione S-transferase P1 gene
transcription regulation in tumor cells
中华医学杂志（英文版）2002，Vol.115Issue(1)：103-106,4.
Effect of trans-acting factor on rat glutathione S-transferase P1 gene transcription regulation in tumor cells
Effect of trans-acting factor on rat glutathione S-transferase P1 gene transcription regulation in tumor cells
摘要
Abstract
Objective To investigate the effect of trans-acting factor(s) on rat glutathione S-transferase P1 gene (rGSTP1) transcription regulation in tumor cells. Methods The binding of trans-acting factor(s) to two enhancers of the rGSTP1 gene, glutathione S-transferase P enhancer Ⅰ (GPEI) and glutathione S-transferase P enhancer Ⅱ-1 (GPEⅡ-1), was identified by an electrophoretic mobility shift assay (EMSA). The molecular weight of trans-acting factor was measured in a UV cross-linking experiment. Results Trans-acting factor interacting with the core sequence of GPEI (cGPEI) were found in human cervical adenocarcinoma cell line (HeLa) and rat hepatoma cell line (CBRH7919). These proteins were not expressed in normal rat liver. Although specific binding proteins that bound to GPEⅡ-1 were detected in all three cell types, a 64 kDa binding protein that exists in HeLa and CBRH7919 cells was absent in normal rat liver. Conclusion cGPEI, GPEII specific binding proteins expressed in HeLa and CBRH7919 cells may play an important role in the high transcriptional level of the rGSTP1 gene in tumor cells.关键词
gene regulation/glutathione S-transferase P1/trans-acting factorKey words
gene regulation/glutathione S-transferase P1/trans-acting factor分类
医药卫生引用本文复制引用
刘东远,廖名湘,左瑾,方福德..Effect of trans-acting factor on rat glutathione S-transferase P1 gene transcription regulation in tumor cells[J].中华医学杂志（英文版）,2002,115(1):103-106,4.基金项目
This work was supported by the National Natural Science Foundation of China (No. 39670173), and “973" Project of China (G1998051016). （No. 39670173）
