安徽农业科学2009，Vol.37Issue(22)：10354-10355,10372,3.
离子萃取分离可见分光光度法测定茶叶中没食子酸含量
Determination on the Gallic Acid Content in Tea by Ionized Extraction Separation and Spectrophotometry
摘要
Abstract
[Objective] The study aimed to establish the method of measuring the gallic acid content in tea by ionized extraction separation and spectrophotometry. [Method] With the Ya′an Zang tea as the sample,the water extract from tea samples was prepared by using water extract method. First,the NaHCO3 was added into the water extract from tea samples to make the ionization treatment,then through the extraction and separation by using ethyl acetate the disturbance factor of gallic acid and catechin were separated out,at last,the content of gallic acid in the water layer of extraction raffinate phase was measured by using spectrophotometer. [Result] The content of gallic acid in the tea sample was (1.32±0.18)% and the relative deviation was 1.36%. The measured value accorded to the reported value in literature and the values of 5 parallel determinations was close each other. The recovery of standard addition in this measured method was 99.4%-103.2%,the RSD value for 3 repeations were lowed than 2.5%. [Conclusion] Compared with other experimental method,the method has obvious advantages such as simple operation,low cost and low requirement of instrument and had higher precision and accuracy in detecting the gallic acid content in tea sample.关键词
茶叶/没食子酸含量/分光光度法/分离Key words
Tea/Gallic acid/Spectrophotometry/Separation分类
化学化工引用本文复制引用
郭俊凌,寇鹏骐,卿钰,李成亮,郑怡萌,杜晓..离子萃取分离可见分光光度法测定茶叶中没食子酸含量[J].安徽农业科学,2009,37(22):10354-10355,10372,3.基金项目
四川农业大学"国家大学生创新型实验计划"基金资助项目(081062638). （081062638）
