安徽农业科学2009，Vol.37Issue(22)：10400-10402,3.
利用改进的整体染色与透明技术观察垂柳的幼胚
The Use of a Improved Whole Stain-clearing Technique for Observations on Younger Embryos of Salix babylonica L.
摘要
Abstract
The paper give a improved whole stain-clearing technique to observed the younger embryos of S. babylonica. After isolated with gum block and stained by diluted Ehrlich's hematoxylin for a proper time, the fertilized ovules were washed with 0.9% sodium chloride (or water), dehydrated with mixed solvent of absolute alcohol and a few immersion oil (artificial cedar oil), and then cleared and mounted in immersion oil. Using an ordinary bright-field microscopy, the younger embryos inside the fertilized ovules were clearly visible. One torpedo-stage embryo with three cotyledons was found. By contrast with Ehrlich's hematoxylin-methyl salicylate technique, the method used immersion oil replace the methyl salicylate (poisonous and difficult to buy), and used a simple procedure to wash and dehydrate the stained ovules. This could finish the mounting at about 20 minutes and had better observing results. The paper also discussed the method of whole stain-clearing technique.关键词
垂柳/胚珠/透明剂/整体透明/植物胚胎学Key words
Salix babylonica L., ovule/Clearing solvent/Whole clearing/Plant embryology分类
农业科技引用本文复制引用
洪亚平,张亚冰..利用改进的整体染色与透明技术观察垂柳的幼胚[J].安徽农业科学,2009,37(22):10400-10402,3.基金项目
国家自然科学基金(30770124) （30770124）
河南科技大学SRTP项目. （）
