首页|期刊导航|南京医科大学学报（英文版）|The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines
南京医科大学学报（英文版）2006，Vol.20Issue(2)：104-108,5.
The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines
The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines
摘要
Abstract
Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4expression in prostate carcinoma cell lines. Methods: Small interference RNA (siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines(PC-3m and LNCaP)with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81% ± 10.20% ,56.10% ± 9.32%at the 24th hour and the 48th hour, compared with 56.93% ±8.78% ,49.24% ± 11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71% ± 6.68% ,58.66% ± 11.56% respectively. Conclusion: The expression of CXCR4 gene can effectively he inhibited by RNAi expression vectors.关键词
RNA interference(RNAi)/CXC receptor 4/expression vector/RT-PCR/western blotKey words
RNA interference(RNAi)/CXC receptor 4/expression vector/RT-PCR/western blot分类
医药卫生引用本文复制引用
..The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines[J].南京医科大学学报（英文版）,2006,20(2):104-108,5.基金项目
National Natural Science Foundation of China(30300348) （30300348）
