安徽农业科学2009，Vol.37Issue(22)：10413-10414,10419,3.
利用改良CTAB法提取淮山叶片高质量DNA
Extraction of High Quality DNA from Leaves of Dioscorea oposetap Thunb. with Modified CTAB Method
摘要
Abstract
[Objective] The study was to provide the basis for molecular biology research on Dioscorea oposetap Thunb. [Method] With young leaves of D. oposetap as the materials,their DNA was extracted with modified CTAB method. The quality and concn. of the extracted DNA were detected by agarose gel electrophoresis and ISSR-PCR amplification. The extracting effect of modified CTAB method on DNA was studied. [Result] The molecular weight of the extracted DNA was similar with that of λDNA. There was 1 clear DNA strip in each lane,and the integrity of the strip was better without protein and RNA pollution and DNA degradation phenomenon. The stable amplified strips could be obtained through ISSR-PCR amplification with template of the extracted DNA. [Conclusion] The modified CTAB method was simple with high efficient,and the quality of DNA extracted by this method was higher,the digestion of RNA was complete,and there were no obvious degradation phenomenon of DNA,and the strip was clear with better polymorphism in ISSR analysis.关键词
淮山/基因组DNA/改良CTAB法Key words
Dioscorea oposetap Thunb/Genome DNA/Modified CTAB method分类
农业科技引用本文复制引用
李月仙,黄东益,黄小龙,周鑫,程文杰,罗欣..利用改良CTAB法提取淮山叶片高质量DNA[J].安徽农业科学,2009,37(22):10413-10414,10419,3.基金项目
高等学校博士学科点专项科研基金(00805650004). （00805650004）
