安徽农业科学2009，Vol.37Issue(22)：10415-10416,10445,3.
小麦不同部位DNA提取的比较研究
Comparative Study on Extraction of Genomic DNA from Different Parts of Wheat
摘要
Abstract
[Objective] The aim was to discuss the effect of different sampling positions of wheat on the DNA quality and yield. [Method] With seeds, root tips and young leaves of wheat as tested materials, the genome DNA from 3 parts of wheat were extracted with modified CTAB method and examined with 0.8% agarose gel electrophoresis. The absorbance value of DNA from 3 parts was measured by ultraviolet spectrophotometer at A260 and A280 and the ratio of A260/A280 was used to determine the DNA purity and the mass concn. DNA isolated from 3 parts of wheat was used as the template for RAPD amplification. [Result] The yield of DNA extracted from wheat leaves was highest, that from the seeds was second, and that from the root tips was lowest. The DNA purity extracted from 3 parts of wheat was similar. The DNA concn. isolated from wheat leaves was the largest, being 756 μg/ml, followed by that from seeds, being 233 μg/ml that from the root was the lowest, being 90 μg/ml. The DNA template extracted from different parts of wheat had no significant effect on RAPD amplification and DNA concn. could achieve the amplification requirements. [Conclusion] The DNA qualities extracted from 3 parts of wheat were good and could be used in subsequent experiments such as digestion and PCR amplification.关键词
小麦/CTAB法/DNA提取/RAPDKey words
Wheat/CTAB/DNA extraction/RAPD分类
农业科技引用本文复制引用
杨培浩,姚大年,张文明,周立人..小麦不同部位DNA提取的比较研究[J].安徽农业科学,2009,37(22):10415-10416,10445,3.基金项目
国家自然科学基金项目(30671301). （30671301）
