中华医学杂志（英文版）2002，Vol.115Issue(7)：1043-1047,5.
逆转录-聚合酶链反应检测滑膜肉瘤石蜡包埋组织中SYT-SSX融合基因
Detection of SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction
魏永昆 1王坚 1朱雄增 1施达仁 1久(冈)正典 2桥本洋2
作者信息
- 1. 复旦大学肿瘤医院病理科,上海,200032
- 2. 产业医科大学医学院病理与肿瘤系,北九州市,807-8555,日本
- 折叠
摘要
Abstract
Objective To assess the feasibility of detecting SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction (RT-PCR).Methods RT-PCR was used to amplify the SYT-SSX fusion transcripts using archival formalin-fixed paraffin-embedded tumor specimens from a series of 37 synovial sarcoma cases. To investigate the specificity of the SYT-SSX fusion transcripts, a variety of non-synovial sarcoma tumors were included in the study as negative controls. The detected messages derived from fusion genes were confirmed by subsequent sequence analysis. Results SYT-SSX fusion transcripts were detected in 33 of 37 (89.2%) synovial sarcomas. None of the 34 cases of non-synovial sarcoma tumors showed amplified products of SYT-SSX fusion transcripts, although PBGD mRNA was detected in all specimens. Among 33 SYT-SSX-positive synovial sarcomas, 22 tumors had an SYT-SSX1 fusion transcript, whereas 6 tumors had an SYT-SSX2 fusion transcript. Fusion types can not be distinguished in the remaining 5 cases. There was a significant relationship between SYT-SSX fusion type and histologic subtype. All 10 biphasic synovial sarcomas had the SYT-SSX1 fusion, whereas all tumors with SYT-SSX2 were of monophasic morphology (P<0.05).Conclusions RT-PCR can be applied to archival formalin-fixed paraffin-embedded tumor tissues as a sensitive and reliable technique for the diagnosis and differential diagnosis of synovial sarcoma. There is an association between SYT-SSX fusion type and histological subtype. SYT-SSX2 fusion transcripts can only be found in monophasic synovial sarcoma.关键词
软组织肿瘤 滑膜肉瘤 SYT-SSX融合基因 RT-PCRKey words
soft tissue tumor/synovial sarcoma/SYT-SSX fusion transcripts/RT-PCR分类
医药卫生引用本文复制引用
魏永昆,王坚,朱雄增,施达仁,久(冈)正典,桥本洋..逆转录-聚合酶链反应检测滑膜肉瘤石蜡包埋组织中SYT-SSX融合基因[J].中华医学杂志（英文版）,2002,115(7):1043-1047,5.
