中国农业科学（英文版）2008，Vol.7Issue(9)：1046-1051,6.
Establishment and Optimization of the Regeneration System of Mature Embryos of Maize (Zea mays L.)
Establishment and Optimization of the Regeneration System of Mature Embryos of Maize (Zea mays L.)
ZHAO Cheng-hao 1ZHANG Li-jun 1GE Chao 1HU Kai1
作者信息
- 1. College of Biotechnology,Shenyang Agricultural University,Shenyang 110161,P.R.China
- 折叠
摘要
Abstract
A reliable system was developed for regeneration from mature embryos derived from callus of four maize inbred lines (Liao 7980,Dan 9818,Dan 340,and Dan 5026).The protocol was mainly based on a series of experiments involving the composition of culture medium.We found that 9 μM 2,4-dichlorophenoxyacetic acid in MS medium was optimum for the induction of callus.The induction frequency of primary calli was over 85% for four inbred lines tested.The addition of L-proline (12 mM) in subculture medium significantly promoted the formation of embryogenic callus but it did not significantly enhance growth rate of callus.Efficient shoot regeneration was obtained on regeneration medium containing 2.22 μM 6-benzylaminopurine in combinations with 4.64 μM Kinetin.Regenerated shoots were rooted on half-strength MS medium containing 2.85 μM indole-3-butyric acid.This plant regeneration system provides a foundation for genetic transformation of maize.关键词
maize (Zea mays L.)/mature embryo/callus induction/embryogenic callus/plant regenerationKey words
maize (Zea mays L.)/mature embryo/callus induction/embryogenic callus/plant regeneration分类
农业科技引用本文复制引用
ZHAO Cheng-hao,ZHANG Li-jun,GE Chao,HU Kai..Establishment and Optimization of the Regeneration System of Mature Embryos of Maize (Zea mays L.)[J].中国农业科学（英文版）,2008,7(9):1046-1051,6.
