分析化学2009，Vol.37Issue(7)：1049-1052,4.
壳聚糖固定化葡萄糖氧化酶生物传感器测定葡萄糖的含量
Determination of Glucose with Biosensor by Immobilization of Glucose Oxidase with Chitosan
摘要
Abstract
A glucose biosensor was fabricated from a glucose oxidasE-immobilized by chitosan and oxygen electrode. The effects of concentration of chitosan(0.3%), enzyme loading(0.8 mg), pH 7.0, phosphate buffer concentration(300 mmol/L), and temperature 25 ℃ for the response of the biosensor were investigated. The glucose biosensor has a linear response range of 0.016-1.10 mmol/L with a detection limit of 8.0 μmol/L(S/N=3). The response time was less than 60 s. The biosensor showed extremely good stability with a shelf-life of at least 3 months. The biosensor exhibited good repeatable response to a 0.25 mmol/L glucose olution with a relative standard deviation of 2.5%(n=10). The precision of fabrication of the biosensors using four different membranes was good with a RSD of 4.7%. Some common potential components in sample such as niacinamide, vitamin B6, vitamin B12, vitamin E, Ca2+, Mg2+, K+ and Zn2+ showed no interferences on the response of the glucose biosensor. The biosensor was successfully applied to determine the glucose in commercial beverage samples.关键词
壳聚糖/葡萄糖氧化酶/葡萄糖/生物传感器/鸡蛋膜Key words
Chitosan/glucose oxidase/glucose/biosensor/eggshell membrane分类
化学化工引用本文复制引用
张彦,南彩凤,冯丽,张利琴,董川,双少敏..壳聚糖固定化葡萄糖氧化酶生物传感器测定葡萄糖的含量[J].分析化学,2009,37(7):1049-1052,4.基金项目
本文系国家自然科学基金重点项目(No.50534100)资助 （No.50534100）
