生命科学研究2007，Vol.11Issue(2)：105-109,5.
一株嗜酸硫氧化菌的分离
Isolation of an Acidophilic Sulfur Oxidizing Bacterium
摘要
Abstract
An acidophilic sulfur-oxidizing bacterium DX-2 was isolated from Dexing area, Jiangxi province in China using enrichment medium and then purified on overlaid solid medium. The morphological, biochemical and physiological characters of DX-2 were studied. The cells of the strain DX-2 were Gram negative and rod-shaped (0.4～0.5) μm by (1～2) μm. The strain was capable of growth by using elemental sulfur or sodium thiosulfate as the sole energy sources. The strain was unable to oxidize ferrous iron. A phylogenetic tree was constructed by comparing published 16S rRNA sequences of the relative bacteria species. In the phylogenetic tree, DX-2 was the closest relative to Acidithiobacillus caldus with more than 99% sequence similarity. The effect of different heavy metals (Cu2+, Cr3+, Cd2+, Sn2+, Pb2+) on growth inhibition of DX-2was investigated.关键词
喜温硫杆菌/16S rRNA/分离/特性/重金属Key words
A cidithiobacillus caldus/16S rRNA/isolation/characterization/heavy metals分类
生物科学引用本文复制引用
程海娜,胡岳华,高健..一株嗜酸硫氧化菌的分离[J].生命科学研究,2007,11(2):105-109,5.基金项目
国家重大基础研究(973)计划项目(2004CB619204) （973）
国家创新研究群体科学基金项目(50621063) Supported by National Basic Research Program(973 Program) of China (2004CB619204),the Chinese Science Foundation for Distinguished Group( 50321402 ). （50621063）
