中华医学杂志（英文版）2001，Vol.114Issue(10)：1060-1063,4.
APP770转基因小鼠的空间学习记忆能力出现减退
Spatiallearning deficits in amyloid precursor protein 770 transgenic mice
李辉 1沈孝宙 1陈永福2
作者信息
- 1. 中国科学院动物研究所
- 2. 中国农业大学生物学院动物生理生化系
- 折叠
摘要
Abstract
To determine whether leaming deficits could be seen in transgenic mice expressing human
amyloid precursor protein 770 (APP770).
Methods Female heterozygous transgenic and nontransgenic mice aged 3, 6 and 9 months at the start of
testing were used, with eight mice in each age group. All mice were subjected to various behavioral tasks
including the Y-maze task and the Morris water maze. After behavioral testing, the mice were sacrificed,
and their brain tissues were used for measuring the choline acetyltransferase (CHAT) activity.
Results Nine-month-old transgenic mice exhibited spatial learming deficits in the Morris water maze and in
spontaneous altemation in the Y-maze, compared with those of the age-matched non-transgenic mice. The
behavioral changes accompanied a reduction of ChAT activity in the cortical and hippocampal regions of
transgenic mice. On the other hand, these behavioral deficits were not observed in transgenic mice either
at 3 or at 6 months of age, in which ChAT activity remained unchanged.
Conclusions The present results show that the learning impairment observed in 9-month-old APP770
transgenic mice are accompanied by a decrease in cortical and hippocampal ChAT activities. This suggests
that cholinergic deficits may be involved in the leaming impairment observed in these APP770 mice. This
model will be a useful tool in advancing our understanding of the relationship between the cholinergic system
and the cognitive deficits observed in Alzheimer's disease (AD).
State Key Laboratory of Reproductive Biology (Li H), Department of
Molecular Biology and Biotechnology (Shen XZ), Institute of Zoology,
The Chinese Academy of Sciences, Beijing 100080, China关键词
阿尔茨海默症胆碱能系统学习记忆转基因分类
医药卫生引用本文复制引用
李辉,沈孝宙,陈永福..APP770转基因小鼠的空间学习记忆能力出现减退[J].中华医学杂志（英文版）,2001,114(10):1060-1063,4.
