草业学报2003，Vol.12Issue(2)：106-111,6.
AMV和WCMV在转基因红三叶中的基因累集
The AMV and WCMV gene pyramiding in transgenic red clover
赵桂琴 1Roger KALLA2
作者信息
- 1. 兰州大学生命科学学院,甘肃,兰州,730000
- 2. 甘肃农业大学草业学院,甘肃,兰州,730070
- 折叠
摘要
Abstract
Artificial hybridization was carried out between AMV (alfalfa mosaic virus) and WCMV (white clovermosaic virus) transgenic red clover plants in order to combine both genes in one plant. Almost all the pair crossesset seeds, the germination rate of T1 plants varied among different crosses, with those from 1 copy transgeneparents gave the highest values. Southern hybridization analysis demonstrates that some T1 plants got more thanone copies of transgene, some of them expressed both transgenes. Their resistance to viruses were assessed byvirus challenge and bioassay, which showed that the T1 plants with both transgenes were resistant against bothAMV and WCMV, from which immune plants were generated. Some of plants were only resistant to either AMVor WCMV, some were susceptible in spite of their positive results in Northern hybridization. Compared with genetransformation in transgenic plants, artificial hybridization between different transgenic plants is a simpler, time-saving way for gene pyramiding.关键词
红三叶/转基因植株/分子生物学分析/人工杂交/抗病性Key words
red clover/transgenic plant/molecular biology analysis/artificial hybridization/virus resistance分类
农业科技引用本文复制引用
赵桂琴,Roger KALLA..AMV和WCMV在转基因红三叶中的基因累集[J].草业学报,2003,12(2):106-111,6.
