国际眼科杂志2008，Vol.8Issue(6)：1065-1069,5.
转化生长因子-β2在糖尿病大鼠视网膜中基因表达的研究
Gene expression of transforming growth factor-β2 in retina of diabetic rats
摘要
Abstract
AIM: To detect the gene expression of TGF-β2 in retinas of diabetic rats at different stages, to observe and analyze the effect of TGF-β2 on the retinas of diabetic rats, to explore the role of TGF-β2 in pathogenesis of diabetic retinopathy (DR), and to provide experiment data and experience for further clinic studies.METHODS: Sprague-Dawley (SD) rats were used and retinas were dissected. The total RNA was isolated from which the first strand of cDNA was prepared. Diabetes mellitus was induced by a single intraperitoneal injection of 60mg/kg streptozotocin (STZ) and the rats were held without insulin treatment until sacrifice. Besides, agematched rats treated with saline were used as controls. Tail vein blood glucose was measured after 2 days and rats were considered hyperglycemic if blood glucose reading>16.7mmol/L. Animals with blood glucose level<16.7mmol/L were excluded from the study. The rats were killed at the 4th, 8th, 12th, 16th, 20th and 24th week respectively after hyperglycemic models were established. The retinas were separated and preserved in liquid nitrogen. The expressions of TGF-β2 gene mRNA were detected by reverse transcription PCR (RT-PCR).RESULTS: The RNA of rat retina was integrative enough to be used to further carry out PCR analysis. Compared with control groups, the expression of TGF-β2 mRNA in retinas of diabetic rats was up-regulated at the 4th week, but there was no statistical difference (P>0.05); it was down-regulated at the 8th week, and there was statistical difference (P<0.05); it was also down-regulated at the 12th week, and there was statistical difference (P<0.05); at the 16th week there was no statistical difference (P>0.05); it was up-regulated at the 20th week, but there was no statistical difference (P>0.05); it continued to be up-regulated at the 24th week, and there was statistical difference (P<0.05).CONCLUSION: Since the expression of TGF-β2 mRNA in retinas of diabetic rats was down-regulated at the 8th week and 12th week statistically, up-regulated at the 24th week statistically, it has obviously shown that TGF-β2 was down-and up-regulated through the period of DR. That is, its changes are diphasic with time. It may confirm that TGF-β2, with the characteristic of diphasic regulation, played an important role in DR. It is necessary to study it furthermore.关键词
转化生长因子-β2/基因表达/视网膜/糖尿病大鼠/逆转录聚合酶链反应Key words
transforming growth factor-β2/gene expression/retina/diabetic rat/RT-PCR分类
医药卫生引用本文复制引用
沈炜,柳林..转化生长因子-β2在糖尿病大鼠视网膜中基因表达的研究[J].国际眼科杂志,2008,8(6):1065-1069,5.基金项目
中国国家自然科学基金资助项目(N0. 30271391) （N0. 30271391）
中国上海市卫生局科研项目(N0. 034124) （N0. 034124）
中国上海市卫生系统百名跨世纪优秀学科带头人培养计划(百人计划)基金资助项目(N0. 057)National Natural Science Foundation of China (No. 30271391) （百人计划）
Foundation of Scientific Research Program of Health Bureau of Shanghai City, China (No. 034124) （No. 034124）
Foundation of Training Plan for a hundred Transcentury Excellent Subject Leaders in Shanghai Public Health System, China (No. 057) （No. 057）
