安徽农业科学2009，Vol.37Issue(22)：10681-10683,3.
燕麦分离蛋白提取工艺研究
Studies on Extraction Technology of Separated Oat Protein
摘要
Abstract
[Objective] The study aimed to optimize the extraction technology of separated oat protein. [Method] With the oat provided by Institute of Alpine Crop in Hebei Province as the tested material, the composition of powder oat was determined with the methods of international reference standard. The powder oat was defated by supercritical carbondioxide extraction technique and the optimum technology condition for preparing separated oat protein by alkali extraction and acid precipitation was studied through orthogonal test. [Result] The contents of moisture, ash, grease, protein and starch in oat were 4.99%, 1.93%, 8.38%, 12.21% and 50.23%,resp..The orthogonal test showed that optimum technology condition for preparing separated oat protein by alkali extraction and acid precipitation was follows: the lixiviate liquor to material of 9, the extraction pH value of 10, the extraction temperature of 50 ℃ and the extraction time of 90 min, under which the extraction rate of separated oat protein was 60.73%. The molecular mass circumscription of oat protein determined by SDS-PAGE was from 20 kDa to 43 kDa. [Conclusion] Using this technology for preparing the separated oat protein could get simple and accurate effect with higher extraction rate.关键词
燕麦蛋白/提取工艺/浸提/Folin-酚/SDS-PAGEKey words
Oat protein/Extraction technology/Lixiviate/Folin-phenol/SDS-PAGE分类
轻工纺织引用本文复制引用
曹辉,李蕾,马海乐..燕麦分离蛋白提取工艺研究[J].安徽农业科学,2009,37(22):10681-10683,3.基金项目
国家"十一五"支撑计划项目(2006BAD27B06). （2006BAD27B06）
