首页|期刊导航|中华医学杂志（英文版）|Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis
中华医学杂志（英文版）2003，Vol.116Issue(7)：1070-1073,4.
Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis
Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis
摘要
Abstract
Objective To examine the global effects of Mycobacterium tuberculosis (M.tuberculosis) infection on macrophages. Methods The gene expression profiling of macrophage U937, in response to infection with M.tuberculosis H37Ra, was monitored using a high-density cDNA microarray. Results M.tuberculosis infection caused 463 differentially expressed genes, of which 366 genes are known genes registered in the Gene Bank. These genes function in various cellular processes including intracellular signalling, cytoskeletal rearrangement, apoptosis, transcriptional regulation, cell surface receptors, cell-mediated immunity as well as a variety of cellular metabolic pathways, and may play key roles in M.tuberculosis infection and intracellular survival. Conclusions M.tuberculosis infection alters the expression of host-cell genes, and these genes will provide a foundation for understanding the infection process of M.tuberculosis. The cDNA microarray is a powerful tool for studying pathogen-host cell interaction.关键词
macrophage/Mycobacterium tuberculosis/gene expressionKey words
macrophage/Mycobacterium tuberculosis/gene expression分类
医药卫生引用本文复制引用
徐永忠,谢建平,李瑶,乐军,陈建平,淳于利娟,王洪海..Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis[J].中华医学杂志（英文版）,2003,116(7):1070-1073,4.基金项目
This work was supported by the National Natural Science Foundation of China (No. 30100007) and the National 973 Project (No. G199054104). （No. 30100007）
