首页|期刊导航|中华医学杂志（英文版）|Roles of NHE-1 in the proliferation and apoptosis of pulmonary
artery smooth muscle cells in rats
中华医学杂志（英文版）2002，Vol.115Issue(1)：107-109,3.
Roles of NHE-1 in the proliferation and apoptosis of pulmonary artery smooth muscle cells in rats
Roles of NHE-1 in the proliferation and apoptosis of pulmonary artery smooth muscle cells in rats
摘要
Abstract
Objective To evaluate the roles of Na+/H+ exchanger-1 （NHE-1）in the proliferation and apoptosis of pulmonary artery smooth muscle cells in rats. Methods Twenty Wistar rats were randomized into control group and 3-week hypoxic group. Intracellular pH (pHi) of the smooth muscle was determined with fluorescence measurement of the pH-sensitive dye BCECF-AM， and the expression of NHE-1 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Primary culture of pulmonary artery smooth muscle cells in vitro was performed. In situ cell death detection kit (TUNEL) was used for studying the effect of specific NHE-1 inhibitor-dimethyl amiloride (DMA) on the apoptosis of muscle cells which had intracellular acidification. Results pHi value and NHE-1 mRNA expression of pulmonary artery smooth muscle cells were significantly higher in the hypoxic group than in the control group (P<0.01, P<0.001). DMA elevated the apoptotic ratio remarkably. The effect was enhanced when DMA concentration increased and the time prolonged. Conclusions With the function of adjusting pHi, NHE-1 may play an important role in the proliferation and apoptosis of pulmonary artery smooth muscle cells.关键词
sodium hydrogen exchanger/pulmonary hypertension/vascular smooth muscle/proliferation/apoptosisKey words
sodium hydrogen exchanger/pulmonary hypertension/vascular smooth muscle/proliferation/apoptosis分类
医药卫生引用本文复制引用
姚伟,钱桂生,杨晓静..Roles of NHE-1 in the proliferation and apoptosis of pulmonary artery smooth muscle cells in rats[J].中华医学杂志（英文版）,2002,115(1):107-109,3.基金项目
This work is supported by the National Natural Science Foundation of China (NO. 39870352). （NO. 39870352）
