军事医学科学院院刊2001，Vol.25Issue(2)：107-110,4.
伯氏疟原虫抗本芴醇株抗性转化相关基因的克隆
Cloning of genes of Plasmodium berghei associated with benflumetol resistance
魏晓莉 1李刚 2李国福 1赵京花 1吴德昌 2时云林1
作者信息
- 1. 军事医学科学院微生物学流行病学研究所,
- 2. 军事医学科学院放射医学研究所,
- 折叠
摘要
Abstract
Objective:To isolate genes related to benflumetol resistance in the rodent malaria P.berghei K173 strain.Methods:The mRNA differential display technique was used to identify differentially expressed genes between drug-sensitive P.berghei K173 strain and benflumetol-resistant strain selected under benflumetol pressure.Northern blot was performed in order to determine the genes actually derived from maternal strains.Results:PCR reactions were prepared respectively with twenty-four pairs of primers in each of the strains.Thirty-three differentially displayed genes fragments were cloned and eight of them were sequenced. The eight sequences were novel ESTs and were submitted to GenBank. Homology searching revealed that clone CB52 was slightly homologous to P.falciparum cyclophilin gene. Hybridization confirmed that the gene fragment CB52 was upregulated in the benflumetol-resistant parasite strain, while downregulated in the chloroquine-resistant strain.Conclusions:CB52 fragment was involved in the benflumetol resistance in P.berghei and the genetic mechanisms of drug-resistance to benflumetol and chloroquine were different.关键词
疟原虫,伯氏/本芴醇/氯喹/基因表达/抗药性分类
医药卫生引用本文复制引用
魏晓莉,李刚,李国福,赵京花,吴德昌,时云林..伯氏疟原虫抗本芴醇株抗性转化相关基因的克隆[J].军事医学科学院院刊,2001,25(2):107-110,4.
