首页|期刊导航|中国农业科学(英文版)|Molecular Characteristics of S1 Gene of Infectious Bronchitis Virus Isolated from Chicken Proventriculus
中国农业科学(英文版)2003，Vol.2Issue(1)：107-112,6.
Molecular Characteristics of S1 Gene of Infectious Bronchitis Virus Isolated from Chicken Proventriculus
Molecular Characteristics of S1 Gene of Infectious Bronchitis Virus Isolated from Chicken Proventriculus
摘要
Abstract
Infectious bronchitis virus was isolated from swollen proventriculi of clinically ill chicken. Thesuspected virus samples (2/97, 3/97, 1/98) were adapted in SPF chicken embryos for virus isolation andidentification. All the virus isolates were able to agglutinate chicken erythrocytes after treatment with trypsin,and interfer with the reproduction of Newcastle disease virus in chicken embryos, and have low antigenic relat-edness values with reference positive IBV. The isolates 2/97, 3/97, 1/98 RNAs extracted from the allantoicfluid of inoculated embryonated eggs were converted to cDNA by reverse transcription with 3'-primer of S1gene of (IBV). Polymerase chain reaction (PCR) was performed with two primers which span the S1 gene.Amplified product of 1.93 kb was subjected to EcoR Ⅰ and BamH Ⅰ digestion and the fragments obtainedwere the same as expected size. The PCR product was ligated to pBlueScript-SK (+) vector, and its nucleotidesequence was determined by the dideoxy-mediated chain termination method. Nucleotide sequence analysisshowed 73.6 - 99.7 % homology between the isolated IBV and the IBV strains in GenBank. The homology ofamino acid was 71.4 - 99.4 %.关键词
Chicken, Infectious bronchitis virus, S1 gene, Molecular characteristicsKey words
Chicken, Infectious bronchitis virus, S1 gene, Molecular characteristics分类
农业科技引用本文复制引用
CHENG Li-qin,ZHOU Ji-yong,John Dikki,SHEN Xing-yan,CHEN Ji-gang,ZHANG De-yong..Molecular Characteristics of S1 Gene of Infectious Bronchitis Virus Isolated from Chicken Proventriculus[J].中国农业科学(英文版),2003,2(1):107-112,6.基金项目
This work was supported by the National Na-ture Science Foundation of China (39970030), theNatural Science Foundation of Zhejiang Province(399411), the Science and Technology Commis-sion of Zhejiang Province (991102030). （39970030）
