国际眼科杂志2008，Vol.8Issue(6)：1073-1075,3.
转化生长因子-β受体Ⅰ/Ⅱ在大鼠视网膜中基因表达的定量检测
Expression of transforming growth factor-β type Ⅰ receptor and transforming growth factor-β type Ⅱ receptor in rat retina
摘要
Abstract
AIM: To quantitatively investigate transforming growth factor-β type Ⅰ receptor (TβRⅠ) and transforming growth factor-β type Ⅱ receptor (TβRⅡ) gene expressions in rat retina.METHODS: Sprague-Dawley rats were chosen in this research. Gene expression was detected quantitatively by reverse transcription polymerase chain reaction (RT-PCR) analysis. RESULTS: The expression level of TβRⅠ and TβRⅡ were 0.00034±0.00013 and 0.0001±0.00005, respectively. The expression level of TβRⅠ was obviously higher than that of TβRⅡ in the rat retina with statistical significance (P<0.01). The ratio of TβRⅠ/TβRⅡ was 3.9±1.7.CONCLUSION: Real time quantitative RT-PCR is an effective method to detect differential expression genes in retina. The change of TβRⅠ/TβRⅡ expression may play an important role in the pathogenesis of retinopathy, which could be further investigated in its significance in the development of proliferation retinopathy.关键词
转化生长因子-β受体/实时荧光定量PCR/基因表达/视网膜Key words
TGF-β receptor/quantitative reverse transcription polymerase chain reaction (QRT-PCR)/gene expression/retina分类
医药卫生引用本文复制引用
沈炜,柳林..转化生长因子-β受体Ⅰ/Ⅱ在大鼠视网膜中基因表达的定量检测[J].国际眼科杂志,2008,8(6):1073-1075,3.基金项目
中国国家自然科学基金资助项目(No.30271391) （No.30271391）
中国上海市卫生局科研项目(No.034124) （No.034124）
中国上海市卫生系统百名跨世纪优秀学科带头人培养计划(百人计划)基金资助项目(No.057)National Natural Science Foundation of China (No.30271391) （百人计划）
Foundation of Scientific Research Program of Health Bureau of Shanghai City, China (No.034124): Foundation of Training Plan for a hundred Transcentury Excellent Subject Leaders in Shanghai Public Health System, China (No.057) （No.034124）
