首页|期刊导航|中国农业科学（英文版）|Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves
中国农业科学（英文版）2009，Vol.8Issue(9)：1075-1086,12.DOI:10.1016/S1671-2927(08)60315-6
Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves
Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves
摘要
Abstract
In order to increase vegetable productivity by improving environmental conditions,this article investigates the effects of exogenous silicon on the activities of major antioxidant enzymes and on lipid peroxidation under chilling stress,and it examines whether silicon-induced chilling tolerance is mediated by an increase in antioxidant activity.Cucumis sativus cv.Jinchun 4 was hydroponically cultivated to the two-leaf stage,at which point seedlings were watered with different concentrations of silicon (0,0.1 and 1 mmol L-1) and separately exposed to normal (25/18℃) or chilling (15/8℃) temperatures for six days under low light (100 umol m-2 s-1).Data were collected from the second leaves on the percentage of withering and the levels of endogenous silicon,malondialdehyde (MDA),hydrogen peroxide (H2O2),superoxide radical (O2·-), superoxide dismutase (SOD,EC 1.15.1.1),glutathione peroxidase (GSH-Px,EC 1.11.1.9),ascorbate peroxidase (APX,EC 1.11.1.11),monodehydroascorbate reductase (MDHAR,EC 1.6.5.4),glutathione reductase (GR,EC 1.6.4.2),reduced glutathione (GSH) and ascorbate (AsA).Compared to normal temperatures,chilling resulted in partially withered leaves and increased MDA content.When 0.1 or 1 mmol L-1 exogenous silicon was combined with chilling.the withering of the cucumber leaves was reduced relative to the original chilling treatment,while the endogenous silicon content was increased,antioxidants such as SOD,GSH-Px,APX,MDHAR,GR,GSH,and AsA were more active,and the levels of H2O2,O2·-,and MDA were lower.We propose that exogenous silicon leads to greater deposition of endogenous silicon and thereby increases antioxidant activities and reduces lipid peroxidation induced by chilling.关键词
antioxidant enzyme/chilling/Cucumis sativus/lipid peroxidation/siliconKey words
antioxidant enzyme/chilling/Cucumis sativus/lipid peroxidation/silicon引用本文复制引用
LIU Jiao-jing,LIN Shao-hang,XU Pei-lei,WANG Xiu-juan,BAI Ji-gang..Effects of Exogenous Silicon on the Activities of Antioxidant Enzymes and Lipid Peroxidation in Chilling-Stressed Cucumber Leaves[J].中国农业科学（英文版）,2009,8(9):1075-1086,12.基金项目
This study was supported by the open fund of the State Key Laboratory of Crop Biology,China (200509). （200509）
