国际眼科杂志2008，Vol.8Issue(6)：1076-1078,3.
大鼠视网膜中转化生长因子β1和β2基因表达的定量检测
Quantitative detection of the expression level of transforming growth factor-β1 and-β2 in rat retina with real time PCR
摘要
Abstract
AIM: To quantitatively detect the expression level of transforming growth factor-β1 (TGF-β1) and transforming growth factor-β2 (TGF-β2) genes in the retina of normal rat in order to determine the expression difference of TGF-β1 and TGF-β2 in retina.METHODS: The total RNA was isolated from which the first strand of cDNA was prepared. The mRNA levels of TGF-β1 and TGF-β2 were detected quantitatively by real time polymerase chain reaction (PCR).RESULTS: The mRNA levels of TGF-β1 and TGF-β2 were 0.0008±0.0003 and 0.0378±0.009, respectively. Expression of TGF-β2 was obviously higher than that of TGF-β1 in rat retina with statistical significance (t=12.37, P<0.001). The ratio of TGF-β2/TG-β1 was 55.00±26.61.CONCLUSION: QRT-PCR could specifically and accurately detect gene expression level in rat retina. In retina the TGF-β2 gene was expressed more abundantly than TGF-β1. It is suggested that TGF-β2 play an important role in retina diseases.关键词
视网膜/实时荧光定量PCR/转化生长因子-β/基因表达Key words
retina/quantitative reverse transcription polymerase chain reaction (QRT-PCR)/TGF-β/gene expression分类
医药卫生引用本文复制引用
沈炜,柳林..大鼠视网膜中转化生长因子β1和β2基因表达的定量检测[J].国际眼科杂志,2008,8(6):1076-1078,3.基金项目
中国国家自然科学基金资助项目(No.30271391) （No.30271391）
中国上海市卫生局科研项目(No.034124) （No.034124）
中国上海市卫生系统百名跨世纪优秀学科带头人培养计划(百人计划)基金资助项目(No.057)National Natural Science Foundation of China (N0.30271391) （百人计划）
Foundation of Scientific Research Program of Health Bureau of Shanghai City, China (N0.034124) （N0.034124）
Foundation of Training Plan for a hundred Trans-century Excellent Subject Leaders in Shanghai Public Health System, China (N0.057) （N0.057）
