中国农业科学(英文版)2003，Vol.2Issue(10)：1076-1082,7.
The Sequence Variations of Intron-3 of the α-Amylase Gene in Adzuki Bean
The Sequence Variations of Intron-3 of the α-Amylase Gene in Adzuki Bean
摘要
Abstract
This study describes variation of intron-3 of a-amylase gene from 156 breeds of adzuki beansusing SSCP(single-strand conformation polymorphism)analysis. Based on a-amylase gene structure and se-quence, A pair of PCR primers, F (CCTACATTCTAACACACCCT) and R (GCATATTGTGCCAGTACAAT)were designed to amplify intron-3 fragments of a-amylase gene. 14 variant types were detected, including 13,9, 10, 4 variant types in the wild, weed, locally cultivated and modern brought-up adzuki beans respectively,9, 8, 7 variant types of the wild adzuki beans from Japan, China and Korea respectively, and some other va-riant types in the local adzuki beans from China and Bhutan. 60 % of subjects of cultivated races were found tobe EE type in the experiment. In addition, sequence analysis of intron-3 of α-amylase gene from 8 varianttypes reveals the evolution process of various variant types in adzuki beans.关键词
Adzuki bean (Vigna angularis )/SSCP (single-strand conformation polymorphism)/α-Amylase gene/Intron/VariationKey words
Adzuki bean (Vigna angularis )/SSCP (single-strand conformation polymorphism)/α-Amylase gene/Intron/Variation分类
农业科技引用本文复制引用
JIN Wen-lin,Yamaguchi Hirofumi,Isigami Matiko,Yasuda Kentaro..The Sequence Variations of Intron-3 of the α-Amylase Gene in Adzuki Bean[J].中国农业科学(英文版),2003,2(10):1076-1082,7.基金项目
The study was supported by China Scholarship Council(20811108),Beijing Natural Science Scholarship(6992009) and Technology Development of Beijing Teaching Council. （20811108）
