南方医科大学学报2006，Vol.26Issue(8)：1083-1086,4.
大肠杆菌表达的人血管内皮细胞生长因子的复性与纯化研究
Refolding and purification of recombinant human VEGF-121 expressed as inclusion bodies in Escherichia coli
摘要
Abstract
Vascular endothelial growth factor 121 (VEGF121) was expressed as inclusion bodies by recombinant Escherichia coli. High concentrations of both biomass (46 g dry cell/L) and VEGF121 inclusion bodies (4.5 g/L) were obtained by applying a high-cell-density culture. After the inclusion bodies were washed and dissolved, VEGF121 was refolded at 0.2 mg/ml by ultrafiltration in refolding buffer with a yield of 81%. Renatured VEGF121 was purified by anion chromatography and Sephacry S-100 chromatography with purity higher than 95% and fnal purification yield of 31%. The purified VEGF121 could stimulate the proliferation of human umbilical vein endothelial cells as demonstrated by a biological activity assay.关键词
包涵体/纯化/包涵体/复性/血管内皮细胞生长因子Key words
inclusion bodies/purification/refolding/vascular endothelial growth factor分类
医药卫生引用本文复制引用
胡志明,马骊,周明乾,高基民,王小宁..大肠杆菌表达的人血管内皮细胞生长因子的复性与纯化研究[J].南方医科大学学报,2006,26(8):1083-1086,4.基金项目
Supported by National "973"Development Project for High-TechResearch (2001CB510008), Sci-tech Development Program ofGuangzhou (2003Z2-E0171) and Key Sci-Tech Research Project ofGuangdong Province (2004B31201010) （2001CB510008）
