生命科学研究2009，Vol.13Issue(2)：109-115,7.
磷酸钙纳米介导自杀基因载体的构建及其应用研究
Construction of Calcium Phosphate Nanoparticles Mediating Fusion Suicide Gene yCDglyTK and its Application in vitro
摘要
Abstract
The three expressing plasmids of pcDNA3.1 (-)CVyCDglyTK,pcDNA3.1 (-)CEAyCDglyTK,pcDNA3.1 (-)CMV-yCDglyTK were constructed with carcino-embryonic antigen (CEA) promoter and cytomegalovirus (CMV), CEA promoter, cytomegalovirus (CMV) enhancer, respectively. Calcium phosphate nanoparticles (CPNP) was used as vector to transfer the three plasmids into CEA-positive cells (Lovo) and CEA-negative cells (HeLa),respectively. The results showed that expression of yCDglyTK mRNA in all cells transferred by the three plasmids above,which significantly sensitized the cytotoxicity of prodrug 5-FC;expression of yCDglyTK mRNA in HeLa cells after transfected by CPNP- pCDNA3.1 (-)CMVyCDglyTK,which enhanced sensitivity of 5-FC,but the other plasmids had no expression of yCDglyTK mRNA and 5-FC had no killing effect, pcDNA3.1 (-)CVyCDglyTK might be a promising candidate vector for colon carcinoma gene therapy.关键词
癌胚抗原/纳米载体/自杀基因/基因治疗Key words
carcino-embryonic antigen/nanoparticle vector/suicide gene/gene therapy分类
生物科学引用本文复制引用
张利元,刘霆,颜金鹏,杨魁,余术宜,颜世能,谢薇,陈玉祥..磷酸钙纳米介导自杀基因载体的构建及其应用研究[J].生命科学研究,2009,13(2):109-115,7.基金项目
国家新药95攻关项目(96-901-05-138) （96-901-05-138）
湖南省自然科学基金资助项目(06JJ4121) 95 national new drug research project(96-901-05-138) （06JJ4121）
