作物学报2004，Vol.30Issue(11)：1097-1101,5.
利用SSR标记分析栽培种花生多态性及亲缘关系
Analysis of DNA Polymorphism and Genetic Relationships in Cultivated Peanut(Arachis hypogaea L.)Using Microsatellite Markers
摘要
Abstract
Eleven SSR primer pairs were used to amplify the genomic DNA isolated from 24 peanut genotypes including all 4 market types. Four primers could detected polymorphism and produced a total of 33 alleles. The average number of alleles per locus was 8.25, and up to 13 alleles were found at one locus. The genetic similarity (GS) indexes among 24 cultivated peanut accessions were calculated based on the data from these SSR markers. The value of GS was varied from 0.2 to 1.0, with an average of 0.4788. The results of UPGMA indicated that the majority of 24 accessions could be divided into two groups by subspecies, and most genotypes were clustered by market types. It is no doubt that microsatellites are very useful DNA markers to analyze DNA polymorphism and genetic relationship in cultivated peanut.关键词
SSR/花生/多态性/遗传关系Key words
Microsatellites/Peanut/Polymorphism/Genetic relationship分类
农业科技引用本文复制引用
韩柱强,高国庆,韦鹏霄,唐荣华,钟瑞春..利用SSR标记分析栽培种花生多态性及亲缘关系[J].作物学报,2004,30(11):1097-1101,5.基金项目
Supported by Guangxi Science Foundation(No.Guikejj 0236038 and No.Guikehui 0342003). （No.Guikejj 0236038 and No.Guikehui 0342003）
