中国农业科学（英文版）2002，Vol.1Issue(10)：1100-1104,5.
Cytoplasmic Male Sterility in Maize
Cytoplasmic Male Sterility in Maize
摘要
Abstract
14 isoplasmic and allonuclear cytoplasmic male sterile lines were used as female parents, 8 tester lines as male parents, 101 F1 progenies were obtained. Fertility restoration response of 101 F1 progenies were investigated through field observation and pollen stainability examination under microscope. 14 isoplasmic and allonuclear cytoplasmic male sterile lines were developed by repeated backcross with recurrent male parent lines for more than 8 generations. The result shows: tester line Zifeng1 not only restored the isoplasmic and allonuclear sterile lines of group C backcrossed with Mo17, Yu30 and Heer, but also completely restored the isoplasmic and allonuclear cytoplasm male sterile lines of group T backcrossed with Mo17, HZS , 1792 ,292 and Yu30. Therefore, nuclear background limits the use of Zifeng1 as a tester for identification of cytoplasmic male sterility. Furthermore RFLPs of mitochondrial DNA of 6 isonuclear and alloplasmic cytoplasmic male sterile lines were analyzed with Bam H Ⅰ and Hind Ⅲ restriction endonuclease and mitochondrial DNA probes pBcmH3 and Cox Ⅱ. The same RFLPs were found within sterile cytoplasm of group C, including C,Chuan G, Lei 2 and Lei 3, but a different RFLP pattern was observed among sterile cytoplasm of group S, C,T and the normal cytoplasm. This result suggested that the RFLP markers tightly linked to sterile mitochondrial genes of different groups could be applied in the identifcation of cytoplasmic male sterility.关键词
Zea mays L/Cytoplasmic male sterility/RFLP/FertilityKey words
Zea mays L/Cytoplasmic male sterility/RFLP/Fertility引用本文复制引用
RONG Ting-zhao,LI Wan-chen,CAO Mo-ju ,HU Chang-yuan..Cytoplasmic Male Sterility in Maize[J].中国农业科学（英文版）,2002,1(10):1100-1104,5.基金项目
This work was supported in part by the National Ninth Five-Year Plan for Tackling Key Scientific Problem ( No. 96-002-02-05-07 ) and Sichuan Province for Tackling Key Breeding Problem (No. 96-002-02-06-01). The staff of Maize Research Institute of Sichuan （ No. 96-002-02-05-07 ）
