首页|期刊导航|南京医科大学学报（英文版）|The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively
南京医科大学学报（英文版）2005，Vol.19Issue(3)：111-115,5.
The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively
The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively
摘要
Abstract
Objective: To reveal the special feature of calponin (CaP) on myosins of different states. Methods: Myosin phosphorylation determination, myosin Mg2 +-ATPase measurement and protein binding assay were used in this study. The lowest CaP/myosin ratio used in the assay was 1/10000(mol/mol), which was 10 thousands-fold lower than the CaP/myosin ratio used in previous studies. Results: In the absence of actin, micro-amount of calponin (MAC) stimulated the Mg2+-ATPase activities of myosin in different states slightly but significantly; and more importantly, MAC significantly increased the precipitations of unphosphorylated myosin, Ca2 + -de-pendently and independently phosphorylated myosins by MLCK but not the myosin phosphorylated by PKA. Conclusion: MAC has a high efficient and selective effect on myosin in the absence of actin.关键词
micro-amount of calponin/myosin phosphorylation/myosin Mg2 + -ATPase activity/precipitationKey words
micro-amount of calponin/myosin phosphorylation/myosin Mg2 + -ATPase activity/precipitation分类
医药卫生引用本文复制引用
Jingxian Yang,Shuyuan Li,Guozhu Han,Yuan Lin..The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively[J].南京医科大学学报（英文版）,2005,19(3):111-115,5.基金项目
This work was supported by a grant from the National Natural Science Foundation of China (No.30070203) （No.30070203）
