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基于matK基因序列的高良姜及其混淆品的鉴定

庞启华 严萍 赵翾 赵树进

陕西科技大学学报(自然科学版)2008,Vol.26Issue(4):11-15,5.
陕西科技大学学报(自然科学版)2008,Vol.26Issue(4):11-15,5.

基于matK基因序列的高良姜及其混淆品的鉴定

IDENTIFICATION OF ALPINIA OFFICINARUM HANCE AND ITS ADULTERANT BASED ON MatK GENE SEQUENCES

庞启华 1严萍 2赵翾 1赵树进3

作者信息

  • 1. 华南理工大学生物科学与工程学院,广东,广州,510008
  • 2. 华南师范大学生命科学学院,广东,广州,510631
  • 3. 广州军区广州总医院药学部,广东,广州,510010
  • 折叠

摘要

Abstract

Partial sequences of matK gene were detected to provide information for identification of A. officinarum Hance and A. galanga (L.) Willd.. A total of 21 sites of nucleotide substitutions were observed in 15 specimens of two Alpinia species, A. officinarum Hance and A. galanga (L.) Willd. could be demarcated by observing the nucleotide sequence at positions 12, 211, 291, 411, 440, 618, 631, 684, 686, 847, 844, 921, 954, 988, 1 011, 1 076, 1 103 and 1 116, in other words, the 18 sites were the marker nucleotides for the identification of this two species. However, the sequence variations of partial matK gene are very small in A. officinarum Hance, so nucleotide sites for discrimination of A. officinarum Hance from different production areas were not found. The results of this study could provide base data for construction of quick and convenient identification methods for A. officinarum Hance.

关键词

高良姜/混淆品/matK基因/鉴定

Key words

Alpinia officinarum Hance/adulterant/matK gene/identification

分类

生物科学

引用本文复制引用

庞启华,严萍,赵翾,赵树进..基于matK基因序列的高良姜及其混淆品的鉴定[J].陕西科技大学学报(自然科学版),2008,26(4):11-15,5.

基金项目

广东省科技厅项目(No.19981212) (No.19981212)

陕西科技大学学报(自然科学版)

OACSTPCD

2096-398X

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