中国现代医学杂志2004，Vol.14Issue(17)：11-15,5.
2型糖尿病患者大网膜与皮下脂肪组织抵抗素蛋白表达的研究
Research of resistin protein expression in abdominal omental and subcutaneous adipose tissue in type 2 diabetic patients
许莉军 1张木勋 1杨再刚 1马洪波 1张晶 1周咏明 1张建华1
作者信息
- 1. 华中科技大学同济医学院附属同济医院,内分泌科,湖北,武汉,430030
- 折叠
摘要
Abstract
Objective:To investigate the expression of resistin protein in abdominal omental and subcutaneous adipose tissues in type 2 diabetic patients and the relationship between obesity and diabetes mellitus.Methods: The expression of resistin protein in omental and subcutaneous adipose tissues was measured using Western blot method.Fasting insulin and glucose levels were measured by enzyme immunoassay and glucose oxidase assay respectively to calculate insulin sensitivity index. Height, weight, waist circumference, hip circumference, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured to calculate waist-hip ratio, body mass index (BMI) and body fat percentage (BF%). Results:Resistin protein expression levels in abdominal omental and subcutaneous fat tissues in normal group (57411+1225.59) (arbitrary OD units) were more higher than those in obesity group (41671+884.38) (P＜0.01), type 2 diabetes mellitus without obesity group (42131+1286.15) (P＜0.01) and type 2 diabetes mellitus with obesity group (42677+1751.10) (P＜0.05) respectively, and there was no significant difference among obesity group,type 2 diabetes mellitus without obesity group and type 2 diabetes mellitus with obesity group. The expression of resistin protein in omental fat tissue was compared with that in subcutaneous fat tissue in oneself and no statistical difference was found. Stepwise regression analysis showed that resistin protein expression levels were negatively correlated with WHR, BMI as well as FBG. The equation was Y=116338-38562×WHR-1154.99×BMI-1243.63×FBG. Conclusions: Resistin protein expression levels in abdominal omental and subcutaneous adipose tissues in normal group are more higher than those in obesity group, type 2 diabetes mellitus without obesity group and type 2 diabetes mellitus with obesity group respectively. The negative correlation between resistin expression levels and WHR, BMI, as well as FBG suggests that resistin may not links obesity to diabetes mellitus.关键词
抵抗素/肥胖/2型糖尿病/胰岛素敏感性/体脂分布Key words
resistin/obesity/type 2 diabetes mellitus/insulin sensitivity/body fat distribution分类
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许莉军,张木勋,杨再刚,马洪波,张晶,周咏明,张建华..2型糖尿病患者大网膜与皮下脂肪组织抵抗素蛋白表达的研究[J].中国现代医学杂志,2004,14(17):11-15,5.
