中国当代儿科杂志2005，Vol.7Issue(2)：112-114,3.
儿童急性白血病端粒酶活性与p15基因表达关系的研究
Relationship between telomerase activity and expression of p15INK4b gene in children with acute leukemia
摘要
Abstract
Objective To study the relationship between telomerase activity and pl5INK4b (pl5) expression in the development of childhood acute leukemia (AL). Methods Telomerase activity and p15 expression in bone marrow (BM) of 27 cases of childhood AL were evaluated using a modified telomeric repeat amplification protocol assay and reversepolymerase chain reaction respectively. For comparative analysis, BM from control donors (n = 9) were analyzed.Results At diagnosis, the telomerase activity detected in BM from AL children was significantly higher than that from control donors (34.5 ±37.0 TPG vs 2.4 ± 2.2 TPG, P < 0. 001 ). The p15 expression in BM of AL children was significantly lower than that of the controls (9.8 ± 16.2% vs 45.8 ± 16.9%, P < 0.001 ). No significant correlation was observed between p15 expression and telomerase activity (r = - 0. 01304, P ＞ 0.05). Conclusions The telomerase upregulation and lower expression of p15 gene may play an important role in the development of AL by different mechanisms.关键词
白血病/端粒酶/p15 INK4B/基因表达/儿童Key words
Leukemia/Telomerase/p15INK4B/Gene expression/Child分类
医药卫生引用本文复制引用
文飞球,陈亦欣,曹励之,刘冯,周克英,陈霆..儿童急性白血病端粒酶活性与p15基因表达关系的研究[J].中国当代儿科杂志,2005,7(2):112-114,3.基金项目
Supported by Medical Research Foundation of Guangdong Province (A2001616) and Initiative Scientific Research Foundation of Ministry of Education of P. R. China for oversea returnees. （A2001616）
