解放军医学杂志2001，Vol.26Issue(2)：113-114,2.
新疆维吾尔族人群HIV协同受体CCR5基因突变的检测
DETECTION OF HIV CORECEPTOR CCR5 MUTATION IN UIGUR POPULATION FROM SINKIANG UIGUR AUTONOMOUS REGION
刘明旭 1王福生 1金磊 1洪卫国 1施红 1雷周云 1刘发军 2张战平3
作者信息
- 1. 北京解放军第302医院
- 2. 壮族军区卫生部防疫处
- 3. 解放军第12医院
- 折叠
摘要
Abstract
Whole blood samples were collected from 316 Uigur subjects inhabiting Kashi City, Sinkiang Uigur Autonomous Region, and their genomic DNA was extracted using QIAgen Blood Kit. DNA fragment spanning CCR5Δ32 and CCR5m303 mutation points were amplified by PCR and genotyped by directed electrophoresis and RFLP with HinCII respectively. In all 316 samples only 22 heterozygotes of CCR5Δ32 were found, in accordance with Hardy-Weinberg equilirium. No CCR5m303 mutation was found. No difference existed between male and female individuals. The frequency of CCR5Δ32 in Uigur population is 3.48%, similar to Medi-Asia ethnic groups (higher than that of Chinese Han people and lower than that of Caucasian people), i.e, in continuity regarding district distribution. Due to its slow-down effect on clinical course of AIDS , high frequency of CCR5Δ32 is highly significant in prevention and treatment of AIDS in Uigur population.关键词
HIV协同受体/CCR5Δ32/基因多态性/聚合酶链反应-限制性内切酶片段长度多态性分类
医药卫生引用本文复制引用
刘明旭,王福生,金磊,洪卫国,施红,雷周云,刘发军,张战平..新疆维吾尔族人群HIV协同受体CCR5基因突变的检测[J].解放军医学杂志,2001,26(2):113-114,2.
