福州大学学报（自然科学版）2001，Vol.29Issue(3)：113-115,3.
一种改进的小分子多肽SDS-PAGE方法
An improved SDS-PAGE method for the separation of low molecular weight polypeptides
徐小华 1陈天豹 1李珑 1倪莉 1饶平凡1
作者信息
摘要
Abstract
An improved SDS-PAGE method for the separation of low molecular weight polypeptides is studied in this paper. A continuous buffer of 0.46?mol/L Tris-0.047?mol/L glycine (pH?9.57)+1.0?g SDS，is used as buffer for both separation gel and stacking gel as well as electrode buffer in our method. Polypeptides with molecular weights from 3.5 to 68.0?kD can be satisfactorily separated with good linearity (correlation coefficient r=0.94) between the relative mobility and the logarithm of molecular weight. It is a SDS-PAGE method with the advantage of being the most widely used one with greater ease and lower cost， and it is very useful for the study of enzymatic cleavage products and diseases.关键词
SDS-PAGE/连续缓冲体系/多肽分类
生物科学引用本文复制引用
徐小华,陈天豹,李珑,倪莉,饶平凡..一种改进的小分子多肽SDS-PAGE方法[J].福州大学学报（自然科学版）,2001,29(3):113-115,3.
