发光学报2003，Vol.24Issue(2)：113-116,4.
用发光法测定DnaJ类分子伴侣MRJ对虫荧光素酶体外重折叠的促进作用
Luminescence Assays for the Luciferase Refolding Facilitated by Human Chaperone DnaJ Homologue MRJ in Vitro
摘要
Abstract
A set of specified protein, the molecular chaperones, could bind with other proteinswhich are not in native state and could promote them to achieve their functional conformations.MRJ (Mouse Related J-protein) is a kind of newly discovered chaperones and is the homologue ofhuman DnaJ. By using denatured luciferase as a molecular model to study the protein refolding andrenaturation processes, the authors incubated the denatured luciferase with three kinds of chaper-ones: MRJ, Hsp60, Hsp70, and their different combinations, respectively. Then, the biolumines-cence from luciferin-ATP-Mg system, which catalyzed by differently treated luciferase, was mea-sured. The results showed that all these chaperones, MRJ, Hsp60 and Hap70, could somewhat in-crease the luminescence from the systems. But only the combination of these three chaperones didhave the most significant and most stable effects. The authors also found the whole processes wereobviously ATP-dependent. From the experiment the author concluded that (1) As a molecularchaperone, MRJ could facilitate the refolding of denatured luciferase; (2) Luminescence assay wasa sensitive, accurate and quick method for the chaperone studies.关键词
分子伴侣/MRJ/蛋白质折叠/发光分析Key words
molecular chaperone/MRJ/protein refolding/luminescence assay分类
生物科学引用本文复制引用
刘亚宁,朱美财,刘成刚,赵新华,陈涛..用发光法测定DnaJ类分子伴侣MRJ对虫荧光素酶体外重折叠的促进作用[J].发光学报,2003,24(2):113-116,4.基金项目
Project supported by National Natural Science Foundation of China (69871031) （69871031）
