动物学研究2007，Vol.28Issue(2)：113-118,6.
利用COI基因序列分析长江与澜沧江水系日本沼虾群体的遗传结构
Genetic Structure of the Oriental River Prawn (Macrobrachium nipponense) from the Yangtze and Lancang Rivers, Inferred from COI Gene Sequence
摘要
Abstract
This study analyzed nucleotide sequences from the mitochondrial cytochrome oxidase submit (COI) gene region (450bp) to investigate the genetic structure of the oriental river prawn (Macrobrachium nipponense) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h=1.000, π=0.028), whereas the population from Chongqing exhibits the lowest level of variability (h=0.700, π=0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (NJ) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.关键词
日本沼虾/COI基因/遗传结构/遗传变异/单倍型Key words
Macrobrachium nipponense/COI gene/Genetic structure/Genetic variation/Haplotype分类
生物科学引用本文复制引用
杨频,张浩,陈立侨,叶金云,禹娜,顾志敏,宋大祥..利用COI基因序列分析长江与澜沧江水系日本沼虾群体的遗传结构[J].动物学研究,2007,28(2):113-118,6.基金项目
This project was supported partly by Key Research Program of Zhejiang (2005C12006-01, 2006C12005) （2005C12006-01, 2006C12005）
