白求恩医科大学学报2001，Vol.27Issue(2)：114-116,3.
HIV-1 gag-gp120嵌合基因重组杆状病毒载体的构建
Construction of recombinant baculovirus vector of HIV-1 gag-gp120 chimeric gene
摘要
Abstract
Objective:A new recombinant baculovirus transfer vector was constructed, in whic h a recombinant gene fragment encoding HIV-1 gag-gp120 chimeric gene was inse rted.Methods:After HIV-1 gag gene and gp120 gene were linked,the recombinant baculov irus vector was constructed,and the DNA recombinant technique and the E coli /baculovirus system were used.Results:Gel electrophoresis of DNA analysis showed that the genes were recombine d correctly.Electron microscope showed that the recombinant baculovirus reproduc ed in a great quantity.Conclusion:Recombinant baculovirus vector which HIV-1 gag-gp120 chimeric gene fragment was inserted in was constructed successfully.This vector is useful in study of the expression and the biological function of the HIV-1 Gag-gp120 ch imeric protein.关键词
人Ⅰ型免疫缺陷病毒/嵌合基因/载体分类
生物科学引用本文复制引用
张东威,金宁一,王宏伟,张应玖,王莉馨,罗坤,李萍,于洪臣,殷震..HIV-1 gag-gp120嵌合基因重组杆状病毒载体的构建[J].白求恩医科大学学报,2001,27(2):114-116,3.基金项目
国家杰出青年基金资助项目 （）
