耳鼻咽喉-头颈外科2001，Vol.8Issue(2)：114-117,4.
bcl-xs增加鼻咽癌细胞对喜树碱诱导凋亡敏感性的实验研究
bcl-xs mediated sensitization of human nasopharyngeal carcinoma cells to camptothecin-induced apoptosis
张月飞 1陈扬超 2周克元 2梁统2
作者信息
- 1. 广东湛江广东医学院附属医院耳鼻咽喉科
- 2. 广东湛江广东医学院生化与分子生物学研究所
- 折叠
摘要
Abstract
Objective: In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.关键词
鼻咽癌/喜树碱/细胞凋亡分类
医药卫生引用本文复制引用
张月飞,陈扬超,周克元,梁统..bcl-xs增加鼻咽癌细胞对喜树碱诱导凋亡敏感性的实验研究[J].耳鼻咽喉-头颈外科,2001,8(2):114-117,4.
