中国临床药理学与治疗学2004，Vol.9Issue(10)：1142-1146,5.
吡格列酮对培养的心肌细胞肥大的抑制作用
Inhibitory effect of pioglitazone on hypertrophy of cultured neonatal rat ventricular myocytes induced by high glucose and norepinephrine
周庆峰 1王洪新 1符丽娟 1杨育红1
作者信息
- 1. 锦州医学院药理学教研室,锦州,121001
- 折叠
摘要
Abstract
To study the inhibitory effect of pioglitazone on cultured hypertrophic ventricular myocyte induced by high glucose and norepinephrine (NE).METHODS: Using cultured ventricular myocytes as a model, the protein contentss was assayed with Lowry' s method, the protein synthesis was assayed with [3H] leucine intake method, the cardiomyocytes' volumes were measured by computer photograph analysis system, and the cellular hypertrophy was observed. The contracting frequency was counted by the inverted microscope. RESULTS: Compared with 1 μnol· L-1 verapamil, pioglitazone at the concentration of 1 - 10 μmol· L-1 inhibited the protein contents, cellular protein synthesis and volumes of the ventricular myocytes in a dose dependent manner. Meanwhile, 10 μmol·L-1 pioglitazone and 1 μmol·L-1 verapamil inhibited cellular contracting frequency too. CONCLUSION: Pioglitazone at the concentration of 1 - 10 μmol· L-1 can effectively inhibit hypertrophy of cultured ventricular myocytes induced by high glucose and NE.关键词
吡格列酮/高糖/肥厚/去甲肾上腺素/心肌细胞Key words
pioglitazone/high glucose/hypertrophy/norepinephrine/ventricular myocytes分类
医药卫生引用本文复制引用
周庆峰,王洪新,符丽娟,杨育红..吡格列酮对培养的心肌细胞肥大的抑制作用[J].中国临床药理学与治疗学,2004,9(10):1142-1146,5.
